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Evidence Library · The add-ons

The one add-on with real evidence behind it

Hyaluronate in the transfer medium works. It also raises the twin rate.

This topic exists because a guide that says every add-on is worthless is not being honest, and would not deserve to be believed about the rest.

Adding hyaluronic acid to the fluid the embryo is transferred in, marketed as embryo glue, has been reviewed across 26 studies covering 6,704 participants. Each outcome below rests on a subset of those studies, given in the table:

OutcomeEffectIn absolute terms
Live birth, 10 trials, 4,066 womenRelative risk 1.21 (95% CI 1.10 to 1.31), moderate qualityBaseline 33% becomes 37% to 44%
Clinical pregnancy, 17 studies, 5,247 womenRelative risk 1.16 (95% CI 1.09 to 1.23), moderate qualityIncreased
Multiple pregnancy, 7 studies, 3,337 womenRelative risk 1.45 (95% CI 1.24 to 1.70), moderate qualityIncreased by about 45% in relative terms
Miscarriage, 7 trialsRelative risk 0.96 (95% CI 0.75 to 1.23) in the studies at low risk of biasNo conclusive effect

That live birth result is roughly eight extra live births per 100 women at a 33% baseline, at moderate certainty. It is the strongest positive finding for any add-on in this stage.

Two reasons it is still not simpleThe multiple pregnancy rate rises by 45% in relative terms, and much of the older evidence comes from an era when more than one embryo was transferred routinely. In a single-embryo-transfer practice the benefit may not carry over intact, and the twin harm should be smaller. Neither of those is established.

The UK regulator rates it yellow, meaning conflicting moderate to high quality evidence, which is more cautious than the Cochrane result alone would suggest and reflects exactly this uncertainty.

If a clinic offers you this one, the evidence supports having the conversation. That is more than can be said for most of the others in this stage, and saying so is the point of including it.

Where this comes from:
90. Heymann D, Vidal L, Or Y, Shoham Z. Hyaluronic acid in embryo transfer media for assisted reproductive technologies. Cochrane Database Syst Rev. 2020;9(9):CD007421.
84. Human Fertilisation and Embryology Authority. Treatment add-ons with limited evidence. London: HFEA; published October 16, 2023, last reviewed February 26, 2026.

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